Embryo Freezers

FREEZE CHARTS PRODUCED USING THE BOVet CREATIONS FREEZERS IN THE TAYLOR WHARTON CP65 (NOW CP100) SHIPPER
Arrows are at stem hole changes. Number 5 is 0°C and each line is 1°C (10°C per inch).
Chart speed is 4 inches per hour.

Small Freezer and Stabilizer LN2 @ 0cm. Hold for seeding at stem #7. Freeze @ 11. Avg. Freeze Rate = .65°C/min. Rm T = 76°F

Small Freezer and Stabilizer LN2 @ 0cm. Hold for seeding at stem #7. Freeze @ 11 & 12. Freeze Rate = .57°C/min. Rm T = 79°F

Large Freezer and Stabilizer LN2 @ 3.5cm. Hold for seeding at stem #7. Freeze @ 11 & 12. Avg. Freeze Rate = .6°C/min. Rm T = 80°F

OBSERVATIONS

The CP65 is a small DRY SHIPPER that works very well with the small BOVet Freezer. Some people use it routinely without making a change in the freezer position during the freeze.

A single drop of one hole in the stem around -20°C makes a curve much closer to a straight line.

A fully charged shipper that is topped off with LN2 prior to leaving to the farm will provide enough liquid nitrogen when poured into a thermos for seeding and plunging.

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